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KMID : 0903519930360040310
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1993 Volume.36 No. 4 p.310 ~ p.314
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Quantitation of fructo - and inulo - oligosaccharides by high performance liquid chromatography
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Abstract
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High performance liquid chromatographic method using a TSK-gel amide 80 column and isocratic elution with acetonitrile-water (63 : 35 ;v/v) mixture was used for the separation and the quantitation of fructo (GF2-GF7)- and inulo-oligosaccharides (F2-F4). Retention time of each standard carbohydrate was highly reproducible. Standardization curves obtained by plotting the peak areas against the amounts of each carbohydrate showed very high coefficient of determination(≥0.9884) and similar slopes, and a wide range of y-intercepts. Our results suggest the use of each pure oligosaccharide for its own standardization curve instead of using a certain carbohydrate as an internal standard.
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